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china mouse il 17a  (Elabscience Biotechnology)


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    Elabscience Biotechnology china mouse il 17a
    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), <t>and</t> <t>interleukin-17</t> <t>(IL-17).(O-Q)</t> Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.
    China Mouse Il 17a, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/china+mouse+il+17a/pmc12789773-150-15-20?v=Elabscience+Biotechnology
    Average 95 stars, based on 40 article reviews
    china mouse il 17a - by Bioz Stars, 2026-07
    95/100 stars

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    1) Product Images from "Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy"

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2024.10.030

    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.
    Figure Legend Snippet: Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Techniques Used: Immunofluorescence, Staining, Expressing, Control



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    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), <t>and</t> <t>interleukin-17</t> <t>(IL-17).(O-Q)</t> Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.
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    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), <t>and</t> <t>interleukin-17</t> <t>(IL-17).(O-Q)</t> Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.
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    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Immunofluorescence, Staining, Expressing, Control